Journal: Nature Communications

Article Title: A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage

doi: 10.1038/s41467-017-00737-8

Figure Lengend Snippet: ZIKV-3′UTR-∆10-LAV and ZIKV-3′UTR-∆20-LAV protect rhesus macaques (RMs) from ZIKV infection. a Scheme of immunization of RMs with 10 3 FFU of WT ZIKV strain FSS13025 ( n = 4), ZIKV-3′UTR-∆10-LAV (∆10; n = 4), ZIKV-3′UTR-∆20-LAV (∆20; n = 3), ZIKV-NS1-LAV or PBS sham ( n = 2) via the subcutaneous route. b Viremia was measured at days 2, 3, 4, 5, 7, and 10 post immunization by qRT-PCR. Each colored line represents data from a different animal in each group. The dashed line indicates the limit of detection (LOD) of the assay. c Pre- and post-challenge antibody neutralization titers. On various days post immunization, sera were measured for neutralizing titers using an mCherry ZIKV infection assay. Red arrows indicate challenge with 10 3 FFU of epidemic ZIKV strain PRVABC59 via the subcutaneous route at day 56 post immunization. The number of animals whose antibody neutralization titers increased by ≥ 4-fold after challenge is indicated by symbol “↑” for each experimental group. d Post-challenge viremia. Viremia was measured by qRT-PCR at days 2, 3, 4, 5, 7, and 10 post challenge. All negative samples are plotted at the half value of LOD. Error bars represent SDs

Article Snippet: Animals were housed and cared in accordance with local, state, federal, and institutional policies in an American Association for Accreditation of Laboratory Animal Care-accredited facility at the Bioqual Inc. RMs (3–4/group) were randomized by body weight, gender, and age, and subcutaneously administered with 10 3 FFU of parental WT ZIKV strain FSS13025, ZIKV-3′UTR-∆10-LAV, ZIKV-3′UTR-∆20-LAV, or PBS sham at day 0.

Techniques: Infection, Quantitative RT-PCR, Neutralization

Journal: Nature Communications

Article Title: A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage

doi: 10.1038/s41467-017-00737-8

Figure Lengend Snippet: Safety evaluation of ZIKV-3′UTR-∆20-LAV (∆20) vaccine candidate. a Viral loads in organs of infected A129 mice. Three-week-old A129 mice ( n = 7) were subcutaneously immunized with 10 3 FFU of WT ZIKV FSS13025 ( left panel ) and its derivative ∆20 vaccine candidate ( right panel ). Organs from infected mice were collected and homogenized at days 6 and 10 post infection. The amounts of viruses were quantified on Vero cells using a focus-forming assay. The mean results from seven animals are presented. Bars denote standard errors. The dashed lines indicate the limit of detection (LOD) of the assay. b – f Effect of ∆20 vaccination on the testis. Three-week-old A129 mice ( n = 5) were subcutaneously infected with 1 × 10 3 FFU of WT ZIKV FSS13025 or ∆20 vaccine candidate. At day 28 post infection, animals from each group were analyzed for testis weight ( b ), testis size ( c ), total sperm counts ( d ), motile sperm counts ( e ), and viral RNA load ( f ). Scale bar , 1 mm. g Comparison of neurovirulence of WT ZIKV FSS13025 and ∆20 vaccine candidate in outbred CD-1 mice. One-day-old CD-1 mice ( n = 7–8/group) were injected intracranially with 10–10 4 FFU of WT ZIKV or 10 3 –10 4 FFU of ∆20 vaccine candidate. Surviving mice and total infected animals are indicated. h Analysis of vector competency. Aedes aegypti were fed on artificial blood-meals spiked with 10 6 FFU/ml of WT ZIKV FSS13025 or ∆20 vaccine virus. At day 7 post feeding, individual engorged mosquitoes were assayed for infection by immunostaining of viral protein expression on inoculated Vero cells. The number of infected mosquitos and total number of engorged mosquitoes are indicated. Asterisks indicate significant differences (one-way ANOVA: *** P -value < 0.001; ** P -value < 0.01; * P -value < 0.05). Nonsignificant (n.s.) with P -value > 0.05. All negative samples are plotted at the half value of LOD. Error bars represent SDs

Article Snippet: Animals were housed and cared in accordance with local, state, federal, and institutional policies in an American Association for Accreditation of Laboratory Animal Care-accredited facility at the Bioqual Inc. RMs (3–4/group) were randomized by body weight, gender, and age, and subcutaneously administered with 10 3 FFU of parental WT ZIKV strain FSS13025, ZIKV-3′UTR-∆10-LAV, ZIKV-3′UTR-∆20-LAV, or PBS sham at day 0.

Techniques: Infection, Focus Forming Assay, Comparison, Injection, Plasmid Preparation, Virus, Immunostaining, Expressing

Journal: Current biology : CB

Article Title: Distinct pathways of adaptive evolution in Cryptococcus neoformans reveal a mutation in adenylyl cyclase with trade-offs for pathogenicity

doi: 10.1016/j.cub.2023.08.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Histopathology images from mice infected with Ftc555-1 Parental and M1 strains , HistoWiz , HistoWiz Data: https://app.histowiz.com/shared_orders/897b4aa6-bf6c-49da-9009-7113f766aee9/slides/.

Techniques: Recombinant, Ligation, Sequencing, Illumina Sequencing, Histopathology, Infection, Knock-Out, Plasmid Preparation, CRISPR, Amplification, Software